class: center, middle, inverse, title-slide .title[ # Unix introduction ] .author[ ### Mikhail Dozmorov ] .institute[ ### Virginia Commonwealth University ] .date[ ### 09-20-2023 ] --- ## What is Unix - Unix is a family of operating systems and environments that exploits the power of linguistic abstractions to perform tasks - Unix is not an acronym; it is a pun on "Multics". Multics was a large multi-user operating system that was being developed at Bell Labs shortly before Unix was created in the early '70s. Brian Kernighan is credited with the name - All computational genomics is done in Unix <center><img src="img/unix_plate.jpg" height="280px" /></center> .small[ http://www.read.seas.harvard.edu/~kohler/class/aosref/ritchie84evolution.pdf --- ## History of Unix - Initial file system, command interpreter (shell), and process management started by Ken Thompson - File system and further development from Dennis Ritchie, as well as Doug McIlroy and Joe Ossanna - Vast array of simple, dependable tools that each do one simple task <center><img src="img/Ken_Thompson_(sitting)_and_Dennis_Ritchie_at_PDP-11_(2876612463).jpg" height="260px" /></center> .small[ Ken Thompson (sitting) and Dennis Ritchie working together at a PDP-11 ] --- ## Philosophy of Unix - Vast array of simple, dependable tools - Each do one simple task, and do it really well - By combining these tools, one can conduct rather sophisticated analyses - The Linux help philosophy: "RTFM" (Read the Fine Manual) --- ## Know your Unix - Unix users spend a lot of time at the **command line** - In Unix, a word is worth a thousand mouse clicks <center><img src="https://www.howtogeek.com/wp-content/uploads/2017/03/img_58c0939c2d487.png" height="370px" /></center> --- ## Unix systems - Three common types of laptop/desktop operating systems: Windows, Mac, Linux. - Mac and Linux are both Unix-like! - What that means for us: Unix-like operating systems are equipped with "shells"" that provide a command line user interface. <center><img src="img/itu-unix-linux-1024x576.jpg" height="370px" /></center> --- ## Shell, aka command line, aka terminal - Shell is an interactive environment with a set of commands to initiate and direct computations - Shell encloses the complexity of OS, hence the name - You type in commands - Shell executes them https://en.wikipedia.org/wiki/Unix_shell --- ## Shell, aka command line, aka terminal - The Bourne shell (`sh`) is a shell, or command-line interpreter, for computer operating systems - Developed by Stephen Bourne at Bell Labs, 1976 - `bash` (the Bourne-Again shell) was later developed for the GNU project and incorporates features from the Bourne shell, `csh`, and `ksh`. It is meant to be POSIX-compliant https://en.wikipedia.org/wiki/Stephen_R._Bourne --- ## Most popular types of shell - `bash` - Bourne-Again shell - `tcsh` - TENEX C shell - `zsh` - Z shell - Change shell: `chsh –s /bin/zsh` - `$SHELL` environmental variable has path to the currently used shell --- ## Getting to the command line - Remote access, SSH, **PuTTY** (http://www.chiark.greenend.org.uk/~sgtatham/putty/), **MobaXterm** (https://mobaxterm.mobatek.net/) - **Mac OS X + Xcode development suite** (free, https://developer.apple.com/xcode/) + **X11 server** (free, https://www.xquartz.org/) + **iTerm2** (optional, https://iterm2.com/) - **Ubuntu Linux** (long-term support LTS version, XX.04, http://www.ubuntu.com/download/desktop) --- ## Getting to the command line | Windows users - **Windows Subsystem for Linux**, https://github.com/sirredbeard/Awesome-WSL - **Cygwin**, http://www.cygwin.com/ - **Git Bash**, https://git-for-windows.github.io/ - Boot from a CD or USB (search for "linux usb") - Install the whole Linux systems as a Virtual Machine in **VirtualBox**, https://www.virtualbox.org/ --- ## Getting to the command line | Mac users - "Terminal" is already installed, `bash` shell - Why? Darwin, the system on which Apple's Mac OS X is built, is a derivative of 4.4 BSD-Lite2 and FreeBSD. In other words, the Mac is a Unix system - For X11 (graphics), see XQuartz, http://xquartz.macosforge.org/landing/ - `iTerm2` - a better terminal replacement for Mac, https://www.iterm2.com/ --- ## Obtaining new command-line software - Modern Unix systems have package managers to that download install (free) software for you - On a Mac, **Homebrew** (http://brew.sh/) is a popular package-management system (alternatively, **MacPorts**, https://www.macports.org/) - On Ubuntu, **apt** (https://itsfoss.com/apt-get-linux-guide/) is the standard package manager, with both a command-line and graphical interface available - On Windows, **Cygwin** (https://cygwin.com/install.html) installs everything precompiled through its setup file. Do not delete `setup-x86_64.exe` file after installing Cygwin, explore what Linux tools are available (a lot) --- ## Interacting with shell - Most commands take additional arguments that fine tune their behavior - If you don't know what a command does, use the command `man <command>` - Press `q` to quit the `man` page viewer - Most often, you’ll use `<command> -h` or `<command> --help` - Some commands output help if executed without any arguments --- ## File system: Full vs. relative paths <center><img src="img/file_paths.png" height="330px" /></center> - `cd /` - go to the root directory - `cd /usr/home/jack/bin` - go to the user’s sub-directory - `cd ..` - go to the upper level directory - `cd`, or `cd ~` - go to the user’s home directory - `cd --` - go to the last visited directory --- ## Orienting in the filesystem - `pwd` - print working directory - `ls` - list all files in the current directory - `ls -1` - list files in _one_ column - `ls –lah` - list files in `l`ong, `h`uman readable format, include `a`ll content, user, owner, permissions --- ## Creating, moving, copying, and removing files - `touch <file>` - creates an empty file - `nano <file>` - edit it - `mkdir <dirname>` - creates a directory - `cp <source_file> <target_file>` - copy a file to another location/file - `mv <source_file> <target_file>` - move a file - `rm <file>` - remove a file. If multiple files provided, removes all of them - `rm –r <dirname>` - recursive removal (deletes a directory) --- ## Permissions: `chmod`, `chown` and `chgrp` In Unix, every file and directory has an **owner** and a **group** - `Owner` - is the one who created a file/directory - `Group` - defines rules of file operations and/or permissions - `Every` - user on a Unix machine can belong to one or more groups Every file has **three permission levels** - what the **u**ser can do - what the **g**roup can do - what the **a**ll can do --- ## Understanding ls -lah output ``` -rw-r--r-- 1 mdozmorov staff 205B Dec 19 11:01 BIOS692.2018.Rproj -rw-r--r-- 1 mdozmorov staff 3.5K Dec 18 10:20 BUILD.md -rw-r--r-- 1 mdozmorov staff 470B Dec 19 08:48 README.md -rw-r--r-- 1 mdozmorov staff 2.1K Dec 19 07:51 _config.yml drwxr-xr-x 10 mdozmorov staff 340B Dec 18 10:20 _includes drwxr-xr-x 10 mdozmorov staff 340B Dec 18 10:20 _layouts drwxr-xr-x 7 mdozmorov staff 238B Dec 18 10:29 _posts -rw-r--r-- 1 mdozmorov staff 1.0K Dec 20 15:54 acknowledgements.md ``` --- ## Understanding ls -lah output - The first column tells you about permissions - The very first character in the permissions column tells you what kind of file it is. A **-** means it's a regular file. A **d** means it's a directory - The next nine characters come in three classes, each has three characters. The three classes are `owner`/`group`/`world` permissions - Inside a permission class, **r** means that class can **r**ead the file; **w** means that class can **w**rite the file; **x** means that class can e**x**ecute the file - The second column has the number of files (inside a directory) - The third and fourth columns tell you the owner and group --- ## Finding your files - `find` - lists all files under the working directory (and its subdirectories) based on arbitrary criteria - `find .` - prints the name of every file or directory, recursively. Starts from the current directory - `find . –type f` - finds files only - `find . –type d –maxdepth 1` - finds directories only, at most 1 level down - `find . –type f –name "*.mp3"` - finds only *.mp3 files - `find . -type f -name "README.md" -exec wc -l {} \;` - find files and execute a command on them --- ## Wildcards and patterns - `*` - matches any character - `?` - matches a single character - `[chars]` - matches any character in chars - `[a-zA-Z]` - matches any character between `a` and `z`, including capital letters - `ls *.md` - `ls [Rt]*` --- ## Looking inside files - `cat <file>` - prints out content of a file. If multiple files, consequently prints out all of them (concatenates) - `zcat` - prints out content of gzipped files - `less <file>` - shows the content of the file one screen at a time **Keyboard shortcuts for more command** - `space` - forward, `b` - backward - `g` - go to the beginning, `G` - go to the end - `/<text>` - starts forward search, enter to find next instance - `q` - quit --- ## Chaining commands: pipes - One of the most useful capabilities of Unix is the ability to redirect the STDOUT of one command into the STDIN of another - The `|` (pipe) character feeds output from the first program (to the left of the `|`) as input to the second program on the right. Therefore, you can string all sorts of commands together using the pipe ``` find . | wc -l cat names.txt | sort | uniq -c ``` - Executing one command AFTER another completed successfully: `<command> && <command>` ``` mkdir music && mv *.mp3 music/ ``` --- ## Chaining commands: redirections - Nearly every command in Unix makes use of a convention to have a "standard input" (also called stdin or STDIN, or channel 0) and "standard output" (also called stdout or STDOUT, or channel 1) - There is also a "standard error" (stderr or STDERR, or channel 2) output that is, by convention, reserved for error messages - `find / 2> error.log` - capture STDERR into a file - `find / 2> /dev/null` - suppress STDERR messages - `find / 2>&1` - add STDERR to STDOUT --- ## Chaining commands: redirections - If you want to dump the standard output into a file, use `command > file` (overwrites the file). `command >> file` (appends to the file) - Redirection example: `ls > README.md` - save file list in the current directory into README.md file - Redirection works in another direction: `grep CC0 < License.md` - Or, the content of a commant into another command: `join <(sort file1) <(sort file2)` --- ## Other essential commands {.smaller} | | | |-----------|----------| | head/tail | cut | | for | comm | | sort | echo | | uniq | basename | | wc | dirname | | tr | history | | grep | which | | join | who | | kill | grep | | tar | seq | | gzip | paste | --- ## Statistical command line goodies **data_hacks**, https://github.com/bitly/data_hacks - Command line tools for data analysis - `histogram.py` - `bar_chart.py` - `sample.py` **datamash**, https://www.gnu.org/software/datamash/ - summary statistics - transposing matrixes --- ## Learn more - https://www.tutorialspoint.com/unix/index.htm - Heng Li's "A Bioinformatician's UNIX Toolbox", http://lh3lh3.users.sourceforge.net/biounix.shtml - Bioinformatics one-liners by Stephen Turner, https://github.com/stephenturner/oneliners - Collection of bioinformatics-genomics bash one liners, using awk, sed etc. https://github.com/crazyhottommy/bioinformatics-one-liners - Links and references to many genomics and bioinformatics resources, https://github.com/crazyhottommy/getting-started-with-genomics-tools-and-resources